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The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA658038(MPG Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA658038(MPG Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA658038(MPG Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)

MPG Antibody

CSB-PA658038
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMPG
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Overview

  • Supplier
    Cusabio
  • Product Name
    MPG Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4350
  • Target name
    MPG
  • Target description
    N-methylpurine DNA glycosylase
  • Target synonyms
    AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg, DNA-3-methyladenine glycosylase, 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, CRA36.1 (3-methyl-adenine DNA glycosylase), N-methylpurine-DNA glycosylase, MPG, proliferation-inducing protein 11, proliferation-inducing protein 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29372
  • Protein Name
    DNA-3-methyladenine glycosylase
  • Scientific Description
    Maintenance of DNA sequences is necessary for vertebrates and other life. DNA is under constant stress by a plethora of DNA-damaging agents present in both the environment and within cells. The potentially deleterious effects of DNA lesions in cells are elegantly resolved by sophisticated DNA repair systems, including base excision repair (BER), nucleotide excision repair (NER) and DNA repair methyltransferase (MTase). Methylated bases, such as 3-methyladenine (3MeA) and 7-methylguanine (7MeG) can be formed by agents in the environment and by endogenous cellular processes. Consequently, in the absence of exposure to environmental agents, DNA methylation damage can be incurred on the genomic DNA of normal mammalian cells. DNA N-glycosylases are base excision-repair proteins that locate and cleave damaged bases from DNA as the first step in restoring the sequence.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AAG/MPG using anti-AAG/MPG antibody (A00037). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAG/MPG antigen affinity purified polyclonal antibody (Catalog # A00037) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AAG/MPG at approximately 37 kDa. The expected band size for AAG/MPG is at 33 kDa.
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