More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MPO Antibody
Western Blot analysis of K562 cells using MPO Polyclonal Antibody.
Western Blot analysis of K562 cells using MPO Polyclonal Antibody.
Western Blot analysis of K562 cells using MPO Polyclonal Antibody.

MPO Antibody

CSB-PA005559
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMPO
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    MPO Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4353
  • Target name
    MPO
  • Target description
    myeloperoxidase
  • Target synonyms
    myeloperoxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05164
  • Protein Name
    Myeloperoxidase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
GeneTex
Myeloperoxidase antibodyGTX135125
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetMPO
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti Myeloperoxidase (MPO)GM-4191-CE/IVD
ApplicationsFlow Cytometry
TargetMPO
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-myeloperoxidaseEB11953
ApplicationsWestern Blot, ELISA
TargetMPO
  • SizePrice
Product group Antibodies
MedChem Express
MPO Antibody(Rabbit)HY-P81219
TargetMPO
  • SizePrice
Anti-Myeloperoxidase Antibody
Product group Antibodies
Antibodies.com
Anti-Myeloperoxidase AntibodyA83524
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-MPO [12F3A4C11]Ab03400-10.0
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMPO
  • SizePrice
Figure 1. Western blot analysis of MPO using anti-MPO antibody (A00372-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # A00372-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 55,80-90 kDa. The expected band size for MPO is at 84 kDa.
Product group Antibodies
Boster Bio
Anti-MPO Antibody Picoband(r)A00372-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetMPO
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MPO Antibody144-62885
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetMPO
  • SizePrice
Product group Antibodies
Biosynth
Myeloperoxidase antibody10-7934
  • SizePrice
HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPO Polyclonal Antibody (bs-4943R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
MPO Polyclonal AntibodyBS-4943R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMPO
  • SizePrice