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Western Blot analysis of K562 cells using MPO Polyclonal Antibody.
Western Blot analysis of K562 cells using MPO Polyclonal Antibody.
Western Blot analysis of K562 cells using MPO Polyclonal Antibody.

MPO Antibody

CSB-PA005559
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMPO
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Overview

  • Supplier
    Cusabio
  • Product Name
    MPO Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4353
  • Target name
    MPO
  • Target description
    myeloperoxidase
  • Target synonyms
    myeloperoxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05164
  • Protein Name
    Myeloperoxidase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MPO using anti-MPO antibody (A00372-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # A00372-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 55,80-90 kDa. The expected band size for MPO is at 84 kDa.
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