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ProductsBack/MPO / Myeloperoxidase Antibody (clone 266-6K1)

MPO / Myeloperoxidase Antibody (clone 266-6K1)

LS-C140083
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMPO
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MPO / Myeloperoxidase Antibody (clone 266-6K1)
  • Delivery Days Customer
    23
  • Application Supplier Note
    For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
  • Applications
    Western Blot
  • Applications Supplier
    WB For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    266-6K1
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4353
  • Target name
    MPO
  • Target description
    myeloperoxidase
  • Target synonyms
    myeloperoxidase
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MPO using anti-MPO antibody (A00372-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # A00372-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 55,80-90 kDa. The expected band size for MPO is at 84 kDa.
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