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ProductsBack/MPST antibody [C2C3], C-term

MPST antibody [C2C3], C-term

GTX108274
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMPST
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Overview

  • Supplier
    GeneTex
  • Product Name
    MPST antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.57 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4357
  • Target name
    MPST
  • Target description
    mercaptopyruvate sulfurtransferase
  • Target synonyms
    MST, TST2, TUM1, 3-mercaptopyruvate sulfurtransferase, human liver rhodanese, tRNA thiouridin modification protein 1, testicular tissue protein Li 200
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25325
  • Protein Name
    3-mercaptopyruvate sulfurtransferase
  • Scientific Description
    This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Rydz L, Wróbel M, Janik K, et al. Hypoxia-Induced Changes in L-Cysteine Metabolism and Antioxidative Processes in Melanoma Cells. Biomolecules. 2023,13(10). doi: 10.3390/biom13101491
    Read this paper
  • Zhang J, Cen L, Zhang X, et al. MPST deficiency promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via AKT. Redox Biol. 2022,56:102469. doi: 10.1016/j.redox.2022.102469
    Read this paper
  • Jurkowska H, Wróbel M, Jasek-Gajda E, et al. Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines. Biomolecules. 2022,12(2). doi: 10.3390/biom12020148
    Read this paper
  • Bronowicka-Adamska P, Bentke A, Lasota M, et al. Effect of S-Allyl -L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis. Int J Mol Sci. 2020,21(3). doi: 10.3390/ijms21031090
    Read this paper
  • Wang YH, Huang JT, Chen WL, et al. Dysregulation of cystathionine γ-lyase promotes prostate cancer progression and metastasis. EMBO Rep. 2019,20(10):e45986. doi: 10.15252/embr.201845986
    Read this paper
  • Jurkowska H, Wróbel M, Szlęzak D, et al. New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H(2)S-donor. Amino Acids. 2018,50(6):699-709. doi: 10.1007/s00726-018-2546-2
    Read this paper
  • Bronowicka-Adamska P, Bentke A, Wróbel M. Hydrogen sulfide generation from l-cysteine in the human glioblastoma-astrocytoma U-87 MG and neuroblastoma SHSY5Y cell lines. Acta Biochim Pol. 2017,64(1):171-176. doi: 10.18388/abp.2016_1394
    Read this paper

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Figure 1. Western blot analysis of MPST using anti-MPST antibody (A04609-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPST antigen affinity purified polyclonal antibody (Catalog # A04609-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPST at approximately 33 kDa. The expected band size for MPST is at 33 kDa.
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