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Western Blot analysis of COS7 cells using MRP-S22 Polyclonal Antibody
Western Blot analysis of COS7 cells using MRP-S22 Polyclonal Antibody
Western Blot analysis of COS7 cells using MRP-S22 Polyclonal Antibody

MRPS22 Antibody

CSB-PA003308
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey
TargetMRPS22
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Overview

  • Supplier
    Cusabio
  • Product Name
    MRPS22 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56945
  • Target name
    MRPS22
  • Target description
    mitochondrial ribosomal protein S22
  • Target synonyms
    C3orf5, COXPD5, GIBT, GK002, MRP-S22, ODG7, RPMS22, mS22, small ribosomal subunit protein mS22, 28S ribosomal protein S22, mitochondrial, S22mt, mitochondrial small ribosomal subunit protein mS22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP82650
  • Protein Name
    28S ribosomal protein S22, mitochondrial
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human Hacat whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRPS22 antigen affinity purified polyclonal antibody (Catalog # A10300-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRPS22 at approximately 38 kDa. The expected band size for MRPS22 is at 41 kDa.
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