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WB analysis of A549 cell lysate using GTX54377 MSH2 antibody. Dilution : 1:2000 Loading : 25μg per lane
WB analysis of A549 cell lysate using GTX54377 MSH2 antibody. Dilution : 1:2000 Loading : 25μg per lane
WB analysis of A549 cell lysate using GTX54377 MSH2 antibody. Dilution : 1:2000 Loading : 25μg per lane

MSH2 antibody

GTX54377
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetMSH2
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Overview

  • Supplier
    GeneTex
  • Product Name
    MSH2 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. IP: 1:20 - 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4436
  • Target name
    MSH2
  • Target description
    mutS homolog 2
  • Target synonyms
    COCA1, FCC1, HNPCC, HNPCC1, LCFS2, LYNCH1, MMRCS2, MSH-2, hMSH2, DNA mismatch repair protein Msh2, DNA mismatch repair protein Msh2 transcript, MutS-like 2, mutS homolog 2, colon cancer, nonpolyposis type 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43246
  • Protein Name
    DNA mismatch repair protein Msh2
  • Scientific Description
    This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MSH2 using anti-MSH2 antibody (A00140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # A00140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105 kDa. The expected band size for MSH2 is at 105 kDa.
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