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ProductsBack/MSN Polyclonal Antibody
Western Blot analysis of 293T and A431 cell using MSN Polyclonal Antibody at dilution of 1:500
Western Blot analysis of 293T and A431 cell using MSN Polyclonal Antibody at dilution of 1:500
Western Blot analysis of 293T and A431 cell using MSN Polyclonal Antibody at dilution of 1:500

MSN Polyclonal Antibody

E-AB-10465
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMSN
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Overview

  • Supplier
    Elabscience
  • Product Name
    MSN Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4478
  • Target name
    MSN
  • Target description
    moesin
  • Target synonyms
    HEL70, IMD50, moesin, epididymis luminal protein 70, membrane-organizing extension spike protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26038
  • Protein Name
    Moesin
  • Scientific Description
    Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. Probably involved in connections of major cytoskeletal structures to the plasma membrane. May inhibit herpes simplex virus 1 infection at an early stage.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of MSN using anti-MSN antibody (A00766-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSN antigen affinity purified polyclonal antibody (Catalog # A00766-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSN at approximately 78KD. The expected band size for MSN is at 66KD.
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