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MST1 Recombinant Antibody

BSM-61834R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMST1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MST1 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4485
  • Target name
    MST1
  • Target description
    macrophage stimulating 1
  • Target synonyms
    D3F15S2; DNF15S2; hepatocyte growth factor-like protein; hepatocyte growth factor-like protein homolog; HGFL; macrophage stimulating 1 (hepatocyte growth factor-like); macrophage-stimulating protein; MSP; NF15S2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26927
  • Protein Name
    Hepatocyte growth factor-like protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MST1 using anti-MST1 antibody (A01069-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human MCF-7 cell lysate, Lane 3: human HepG2 cell lysate, Lane 4: human SK-OV-3 cell lysate, Lane 5: human A549 cell lysate, Lane 6: rat kidney tissue lysate, Lane 7: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST1 antigen affinity purified polyclonal antibody (Catalog # A01069-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST1 at approximately 80KD. The expected band size for MST1 is at 80KD.
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