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MT-ND6 Polyclonal Antibody

RD76971A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetND6
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    MT-ND6 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4541
  • Target name
    ND6
  • Target description
    NADH dehydrogenase subunit 6
  • Target synonyms
    MTND6; NADH dehydrogenase subunit 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. The significance of NADH dehydrogenase subunit 6 (ND6) is rapidly becoming increasingly apparent as many mutations leading to amino acid changes in this subunit are associated with known mitochondrial diseases.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ND6/MT-ND6 using anti-ND6/MT-ND6 antibody (A03777-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ND6/MT-ND6 antigen affinity purified polyclonal antibody (Catalog # A03777-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ND6/MT-ND6 at approximately 19 kDa. The expected band size for ND6/MT-ND6 is at 19 kDa.
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