Western blot using GeneTexs anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).
![WB analysis of transfected (+) and non-transfected (-) 293T whole cell lysate using GTX48646 MTBP antibody [PHL-1]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX48646/GTX48646_20240423_WB_246_24042320_179.webp "WB analysis of transfected (+) and non-transfected (-) 293T whole cell lysate using GTX48646 MTBP antibody [PHL-1]. Dilution : 1:500")
Western blot using GeneTexs anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).
MTBP antibody [PHL-1]
GTX48646
Overview
- SupplierGeneTex
- Product NameMTBP Antibody [PHL-1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:2000. ELISA: 1:2000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDPHL-1
- ConjugateUnconjugated
- Gene ID27085
- Target nameMTBP
- Target descriptionMDM2 binding protein
- Target synonymsMDM2 (mouse double minute 2)-binding protein, 104kD; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa; mdm2-binding protein; MDM2BP
- HostMouse
- IsotypeIgG1
- Protein IDQ96DY7
- Protein NameMdm2-binding protein
- Scientific DescriptionThe MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor. Studies also indicate that MDM2 regulates cell prolifer-ation via p53 independent mechanisms. MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1). MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2). The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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