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WB analysis of various sample lysates using GTX66550 MTMR2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66550 MTMR2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66550 MTMR2 antibody. Dilution : 1:1000 Loading : 25microg per lane

MTMR2 antibody

GTX66550
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetMTMR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    MTMR2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8898
  • Target name
    MTMR2
  • Target description
    myotubularin related protein 2
  • Target synonyms
    CMT4B, CMT4B1, phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR2, phosphatidylinositol-3,5-bisphosphate 3-phosphatase, phosphatidylinositol-3-phosphatase, phosphatidylinositol-3-phosphate phosphatase, phosphoinositide-3-phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13614
  • Protein Name
    Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR2
  • Scientific Description
    This gene is a member of the myotubularin family of phosphoinositide lipid phosphatases. The encoded protein possesses phosphatase activity towards phosphatidylinositol-3-phosphate and phosphatidylinositol-3,5-bisphosphate. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4B, an autosomal recessive demyelinating neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of MTMR2 using anti-MTMR2 antibody (A04202-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTMR2 antigen affinity purified polyclonal antibody (Catalog # A04202-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTMR2 at approximately 73 kDa. The expected band size for MTMR2 is at 73 kDa.
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