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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA859939LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA859939LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA859939LA01HU at dilution of 1:100

MTR Antibody

CSB-PA859939LA01HU
Cusabio
ApplicationsImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMTR
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Overview

  • Supplier
    Cusabio
  • Product Name
    MTR Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4548
  • Target name
    MTR
  • Target description
    5-methyltetrahydrofolate-homocysteine methyltransferase
  • Target synonyms
    5-methyltetrahydrofolate-homocysteine methyltransferase 1; cblG; cobalamin-dependent methionine synthase; HMAG; methionine synthase; MS; vitamin-B12 dependent methionine synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99707
  • Protein Name
    Methionine synthase
  • Scientific Description
    Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTR using anti-MTR antibody (A01140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTR antigen affinity purified polyclonal antibody (Catalog # A01140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTR at approximately 140KD. The expected band size for MTR is at 118KD.
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