Immunohistochemical analysis of paraffin-embedded human hepatoma, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mtRNA polymerase antibody [N2N3] (GTX105137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX105137/GTX105137_40408_20240628_WB_24070822_113.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mtRNA polymerase antibody [N2N3] (GTX105137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mtRNA polymerase antibody [N2N3] (GTX105137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX105137/GTX105137_45378_20240426_WB_25100823_294.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mtRNA polymerase antibody [N2N3] (GTX105137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded human hepatoma, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
mtRNA polymerase antibody [N2N3]
GTX105137
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPOLRMT
Overview
- SupplierGeneTex
- Product NamemtRNA polymerase antibody [N2N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5442
- Target namePOLRMT
- Target descriptionRNA polymerase mitochondrial
- Target synonymsAPOLMT, COXPD55, MTRNAP, MTRPOL, h-mtRPOL, DNA-directed RNA polymerase, mitochondrial, polymerase (RNA) mitochondrial (DNA directed)
- HostRabbit
- IsotypeIgG
- Protein IDO00411
- Protein NameDNA-directed RNA polymerase, mitochondrial
- Scientific DescriptionThis gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Thakur J, Henikoff S. Unexpected conformational variations of the human centromeric chromatin complex. Genes Dev. 2018,32(1):20-25. doi: 10.1101/gad.307736.117Read this paper
- Kasinathan S, Henikoff S. Non-B-Form DNA Is Enriched at Centromeres. Mol Biol Evol. 2018,35(4):949-962. doi: 10.1093/molbev/msy010Read this paper
- Sobek S, Dalla Rosa I, Pommier Y, et al. Negative regulation of mitochondrial transcription by mitochondrial topoisomerase I. Nucleic Acids Res. 2013,41(21):9848-57. doi: 10.1093/nar/gkt768Read this paper
Datasheet
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