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ProductsBack/MUC20 Antibody - C-terminal region (OAAB17284)

MUC20 Antibody - C-terminal region (OAAB17284)

OAAB17284
Aviva Systems Biology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMUC20
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    MUC20 Antibody - C-terminal region (OAAB17284)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.45 mg/ml
  • Format
    Liquid. PBS with 0.09% (W/V) sodium azide.
  • Gene ID200958
  • Target name
    MUC20
  • Target description
    mucin 20, cell surface associated
  • Target synonyms
    MUC-20; mucin-20; transmembrane mucin MUC20S
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MUC20 using anti-MUC20 antibody (A07372). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates Lane 2: human A549 whole cell lysates Lane 3: uman HL-60 whole cell lysates Lane 4: uman K562 whole cell lysates Lane 5: rat kidney tissue lysates Lane 6: mouse kidney tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC20 antigen affinity purified polyclonal antibody (Catalog # A07372) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC20 at approximately 75KD. The expected band size for MUC20 is at 75KD.
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