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The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA282680(MUC20 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA282680(MUC20 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA282680(MUC20 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

MUC20 Antibody

CSB-PA282680
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMUC20
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Overview

  • Supplier
    Cusabio
  • Product Name
    MUC20 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200958
  • Target name
    MUC20
  • Target description
    mucin 20, cell surface associated
  • Target synonyms
    MUC-20; mucin-20; transmembrane mucin MUC20S
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N307
  • Protein Name
    Mucin-20
  • Scientific Description
    This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The shorter isoform expressed by this gene is localized to the plasma membrane, whereas the longer isoform might be secreted. The C terminus of this protein associates with the multifunctional docking site of the met proto-oncogene and suppresses activation of some downstream met signaling cascades. The protein features a tandem repeat domain that varies between 2 and 6 copies in most individuals. The allele represented in the human reference assembly contains 12 copies. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MUC20 using anti-MUC20 antibody (A07372). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates Lane 2: human A549 whole cell lysates Lane 3: uman HL-60 whole cell lysates Lane 4: uman K562 whole cell lysates Lane 5: rat kidney tissue lysates Lane 6: mouse kidney tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC20 antigen affinity purified polyclonal antibody (Catalog # A07372) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC20 at approximately 75KD. The expected band size for MUC20 is at 75KD.
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