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MUC4 Monoclonal Antibody

CAB3438
Assay Genie
TargetMUC4
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    MUC4 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4585
  • Target name
    MUC4
  • Target description
    mucin 4, cell surface associated
  • Target synonyms
    ASGP, HSA276359, MUC-4, mucin-4, ascites sialoglycoprotein, mucin 4, tracheobronchial, pancreatic adenocarcinoma mucin, testis mucin, tracheobronchial mucin
  • Host
    Rabbit
  • Protein IDQ99102
  • Protein Name
    Mucin-4
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MUC4 using anti-MUC4 antibody (A01652-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC4 antigen affinity purified polyclonal antibody (Catalog # A01652-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC4 at approximately 170 kDa. The expected band size for MUC4 is at 232 kDa.
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