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Paraformaldehyde-fixed, paraffin embedded rat stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MUC5AC Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MUC5AC Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MUC5AC Polyclonal Antibody, Unconjugated (bs-7166R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

MUC5AC Polyclonal Antibody

BS-7166R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Rat
TargetMUC5AC
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Overview

  • Supplier
    Bioss
  • Product Name
    MUC5AC Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4586
  • Target name
    MUC5AC
  • Target description
    mucin 5AC, oligomeric mucus/gel-forming
  • Target synonyms
    MUC5, TBM, leB, mucin, mucin-5AC, gastric mucin, lewis B blood group antigen, major airway glycoprotein, mucin 5, subtypes A and C, tracheobronchial/gastric, mucin 5AC, oligomeric mucus/gel-forming pseudogene, mucin-5 subtype AC, tracheobronchial, tracheobronchial mucin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98088
  • Protein Name
    Mucin-5AC
  • Reactivity
    Canine, Equine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Taki K, Ohmuraya M, Tanji E, et al. GNAS(R201H) and Kras(G12D) cooperate to promote murine pancreatic tumorigenesis recapitulating human intraductal papillary mucinous neoplasm. Oncogene. 2016,35(18):2407-12. doi: 10.1038/onc.2015.294
    Read this paper