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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA780399(MUC7 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA780399(MUC7 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA780399(MUC7 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

MUC7 Antibody

CSB-PA780399
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMUC7
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Overview

  • Supplier
    Cusabio
  • Product Name
    MUC7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4589
  • Target name
    MUC7
  • Target description
    mucin 7, secreted
  • Target synonyms
    MG2, mucin-7, MUC-7, apo-MG2, mucin 7, salivary, mucin MG2, salivary mucin-7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAX7
  • Protein Name
    Mucin-7
  • Scientific Description
    This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. This antimicrobial protein has antibacterial and antifungal activity. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5 UTR, but encoding the same protein, have been found for this gene.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MUC7 using anti-MUC7 antibody (A05210). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC7 antigen affinity purified polyclonal antibody (Catalog # A05210) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC7 at approximately 46KD. The expected band size for MUC7 is at 39KD.
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