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Mucin 4 (1G8) HRP

SC-33654HRP
Santa Cruz Biotechnology
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMUC4
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Mucin 4 (1G8) HRP
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    HRP
  • Gene ID4585
  • Target name
    MUC4
  • Target description
    mucin 4, cell surface associated
  • Target synonyms
    ASGP, HSA276359, MUC-4, mucin-4, ascites sialoglycoprotein, mucin 4, tracheobronchial, pancreatic adenocarcinoma mucin, testis mucin, tracheobronchial mucin
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: PANC1; Lane 2: LOVO; Lane 3: mouse thymus; Lane 4: MKN45 lysates probed with Mucin 4 Polyclonal Antibody, Unconjugated (bs-1994R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
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Figure 1. Western blot analysis of MUC4 using anti-MUC4 antibody (A01652-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC4 antigen affinity purified polyclonal antibody (Catalog # A01652-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC4 at approximately 170 kDa. The expected band size for MUC4 is at 232 kDa.
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