Multiplex ELISA Kit For Human Malaria (5-Plex)

Boster Bio

Multiplex ELISA Kit For Human Malaria (5-Plex)

9

Research Use Only

Product Overview The Multiplex ELISA Kit For Human Malaria (5-Plex) is a robust and fully quantitative chemiluminescent assay for the concurrent surveillance of four malaria biomarkers. The assay simultaneously measures HRP2, LDH-Pan, LDH-Pv, LDH-Pf, and one host biomarker, CRP. The monitoring of subclinical Malaria infections and prevalence of HRP2-deletions is achieved by the coupling of the most sensitive commercially available HPR2 assay with LDH detection. Validated protocols for whole blood samples are in place, with protocols for dry blood spots in development.Each kit contains a 96-well plate and all other reagents necessary to perform the assay. The individual wells of the plate are precisely and reproducibly arrayed with each of the five biomarkers for accurate results.In 3.5 hours, each kit will report quantities of 80 samples for the four Malaria biomarkers and one host biomarker CRP. This is achieved while only using 15 microl of whole blood per sample. Assay Ranges Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents include: Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Assay Diluent: Liquid, 6 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Calibrator Diluent: Lyophilized, buffered protein solution containing preservatives Competitor: Lyophilized, biotinylated competitive antigen Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Calibrator: Lyophilized, recombinant antigens in a buffered protein base Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Substrate A+: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Plate Seals (3): Adhesive strips

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