Belgium

Luxembourg

Netherlands

Mutagenesis

Molecular Biology

Cosmo Bio USA

Jena Bioscience

Supplier

Research Use Only

Certification

Business Unit

PRX-CLD2331 | Site-Directed Unnatural Mutagenesis | Cosmo Bio USA available at Bio-Connect | Order online now!

Site-Directed Unnatural Mutagenesis | PRX-CLD2331 | Cosmo Bio USA

Site-Directed Unnatural Mutagenesis, AzoAla (amber), CloverDirect

CloverDirect™ tRNA Reagents for Site-Directed Protein Functionalization are designed to permit the incorporation of unnatural amino acids at defined positions within proteins using cell-free coupled transcription/translation systems, including PUREfrex® , PURESYSTEM® and The RYTS Kit. The incorporation positions of unnatural amino acids are defined by selectively introducing UAG amber or CGGG four-base codons into translation templates. For template construction, we recommend Protein Express Linear Template PCR kits (available in two forms: His-tag only or ProX-tag + His-tag) or expression plasmids from Protein Express (pROX-FL92.1: ProX-tag + His-tag) or BioComber (pURE1-4: no tags). The ProX tag is a special N-terminal peptide designed for the incorporation of certain large unnatural amino acids that require precise N-terminal positioning (codon 9) and a specific codon context (flanked by AAC and TCT). Codon 9 of the ProX tag can be varied between UAG, CGGG or TTT (as a control). During cell-free translation, unnatural amino acid-charged aminoacyl-tRNAs recognize either UAG amber or CGGG codons. If a UAG codon is recognized by release factor 1 (one of three termination factors), protein synthesis will be terminated. If the first three nucleotides of CGGG are recognized as a triplet codon by Arg-tRNA, the reading frame shifts to +1 and a downstream stop codon will terminate protein synthesis. Therefore, full-length translation products will contain unnatural amino acids at 100% efficiency. CloverDirect™ offers off-the-shelf UAG or CGGG aminoacyl tRNAs charged with unnatural amino acids that permit: <ol> Fluorescent-labeling Biotin-labeling PEGylation Chemical crosslinking Photo-regulation of a variety of biological processes, including enzyme, receptor, and ion channel activity </ol> In addition, custom services for the expression of proteins with unnatural amino acids are available upon request.

PP-101 | JBS dNTP-Mutagenesis Kit, Random Mutagenesis by dNTP Analogs | Jena Bioscience products available at Bio-Connect

JBS dNTP-Mutagenesis Kit, Random Mutagen... | PP-101 | Jena Bioscience

JBS dNTP-Mutagenesis Kit, Random Mutagenesis by dNTP Analogs

JBS Mutagenesis Series Within three billion years of evolution, nature has produced a plethora of proteins simply by repeated cycles of random mutagenesis followed by in vivo selection for superior function of the encoded proteins. This example of natural evolution has guided researchers within the last two decades to develop strategies for in vitro permutation of proteins. Among the variety of strategies applied, three major powerful techniques have emerged. Random Mutagenesis by dNTP Analogs This method is based on the incorporation of mutagenic dNTP analogs, such as 8-oxo-dGTP and dPTP, into an amplified DNA fragment by PCR. The mutagenic dNTPs are eliminated by a second PCR step in the presence of the four natural dNTPs only, resulting in a rate of mutagenesis of up to 20%. to JBS dNTP-Mutagenesis Kit #PP-101 Random Mutagenesis by Error-Prone PCR Developed by Caldwell & Joyce (1992) this method introduces mutations in the gene of interest using a PCR reaction under conditions that induce an increased error-rate of the DNA-polymerase. The rate of mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0%. to JBS Error-Prone Kit #PP-102 Random Mutagenesis by DNA Shuffling Developed by Stemmer (1994) DNA shuffling generates libraries by random fragmentation of one gene or a pool of related genes, followed by the reassembly of the fragments in a self-priming PCR reaction. This method allows the recombination of sequences from different, related genes. The overall rate of mutagenesis is approx. 0.7%. to JBS DNA-Shuffling Kit #PP-103 Jena Bioscience now offers all components necessary for each of these techniques ready-to-go in a separate kit, accompanied by a streamlined documentation that maximizes success.

PP-103 | JBS DNA-Shuffling Kit, Random Mutagenesis by DNA Shuffling | Jena Bioscience products available at Bio-Connect

JBS DNA-Shuffling Kit, Random Mutagenesi... | PP-103 | Jena Bioscience

JBS DNA-Shuffling Kit, Random Mutagenesis by DNA Shuffling

PP-102 | JBS Error-Prone Kit, Random Mutagenesis by Error-Prone PCR | Jena Bioscience products available at Bio-Connect

JBS Error-Prone Kit, Random Mutagenesis ... | PP-102 | Jena Bioscience

JBS Error-Prone Kit, Random Mutagenesis by Error-Prone PCR

Website

Events

Promotions

Our company

Our team

For suppliers

Vacatures

About us

Headquarters

Customer Service

Technical support

Accountmanagers

Contact

Our people

Antibodies

Chemicals

Proteins / Signaling Molecules

Assays

(Cell) Culture

Products

Our Company

Suppliers

Divisions

News

Bio-Connect

Biotech, Pharma & CRO

Academia

Diagnostics

Environment

Food

Veterinary

Industries

Disclaimer

Privacy Statement

Terms and conditions

Are you looking for specific products, alternatives, documentation or need assistance with solving product related issues? Let our in-house technical support team help you.

We support your research

<p>For 15 years, we connect life science researchers specialized in the import and distribution of life science reagents from global suppliers.</p>

Support your research

DNA / RNA / Vectors

Cell Culture

Genome Editing and Engineering

Lysates / Extracts

Expression

Labware / Accesories

Body Products

Technique Specific Products

Arrays / Slides

Diagnostic

Biologicals

Life Sciences

Services

Become a partner

Call +31 (0)26 326 44 50

Mutagenesis

Didn't find what you were looking for?