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Western Blot Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue All lanes: MVD antibody at 7.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue All lanes: MVD antibody at 7.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue All lanes: MVD antibody at 7.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa

MVD Antibody

CSB-PA015246LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMVD
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Overview

  • Supplier
    Cusabio
  • Product Name
    MVD Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4597
  • Target name
    MVD
  • Target description
    mevalonate diphosphate decarboxylase
  • Target synonyms
    diphosphomevalonate decarboxylase; FP17780; MDDase; mevalonate (diphospho) decarboxylase; mevalonate pyrophosphate decarboxylase; MPD; POROK7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53602
  • Protein Name
    Diphosphomevalonate decarboxylase
  • Scientific Description
    Performs the first committed step in the biosynthesis of isoprenes.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MVD using anti-MVD antibody (A01631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVD antigen affinity purified polyclonal antibody (Catalog # A01631-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVD at approximately 45KD. The expected band size for MVD is at 43KD.
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