Myc tag antibody [GT0002] detects Myc tag protein at cell membrane by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Myc tag stained by Myc tag antibody [GT0002] (GTX628259) diluted at 1:2000.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc tag antibody [GT0002] (GTX628259) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628259/GTX628259_41057_20181005_WB_B_w_23061202_339.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc tag antibody [GT0002] (GTX628259) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Myc tag antibody [GT0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [GT0002] (GTX628259). (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected by GTX628259 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX221667-01) was used in Western blot.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628259/GTX628259_41057_IP_w_23061202_407.webp "Myc tag antibody [GT0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [GT0002] (GTX628259). (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected by GTX628259 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX221667-01) was used in Western blot.")
![Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (GTX628259) (1 μg/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628259/GTX628259_41309_20240621_ELISA_Indirect_24062501_630.webp "Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (GTX628259) (1 μg/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
Myc tag antibody [GT0002] detects Myc tag protein at cell membrane by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Myc tag stained by Myc tag antibody [GT0002] (GTX628259) diluted at 1:2000.
Myc tag antibody [GT0002]
GTX628259
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameMyc tag antibody [GT0002]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. ICC/IF: 1:100-1:2000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT0002
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Gómez R, Viera A, Moreno-Mármol T, et al. Kinase PLK1 regulates the disassembly of the lateral elements and the assembly of the inner centromere during the diakinesis/metaphase I transition in male mouse meiosis. Front Cell Dev Biol. 2022,10:1069946. doi: 10.3389/fcell.2022.1069946Read this paper
- Gómez R, Felipe-Medina N, Ruiz-Torres M, et al. Sororin loads to the synaptonemal complex central region independently of meiotic cohesin complexes. EMBO Rep. 2016,17(5):695-707. doi: 10.15252/embr.201541060Read this paper
- Lin W, Sampathi S, Dai H, et al. Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity. EMBO J. 2013,32(10):1425-39. doi: 10.1038/emboj.2013.88Read this paper