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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 antibody (GTX112987) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 antibody (GTX112987) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MyD88 antibody (GTX112987) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MyD88 antibody

GTX112987
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMYD88
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Overview

  • Supplier
    GeneTex
  • Product Name
    MyD88 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.18 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4615
  • Target name
    MYD88
  • Target description
    MYD88 innate immune signal transduction adaptor
  • Target synonyms
    IMD68, MYD88D, WM1, myeloid differentiation primary response protein MyD88, TLR adaptor MYD88, mutant myeloid differentiation primary response 88, myeloid differentiation primary response 88, myeloid differentiation primary response gene (88)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99836
  • Protein Name
    Myeloid differentiation primary response protein MyD88
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Liang W, Gao Y, Zhao Y, et al. Lactiplantibacillus plantarum ELF051 Alleviates Antibiotic-Associated Diarrhea by Regulating Intestinal Inflammation and Gut Microbiota. Probiotics Antimicrob Proteins. 2024,16(6):1996-2006. doi: 10.1007/s12602-023-10150-x
    Read this paper
  • Liu G, Lin J, Zhang L, et al. Uncovering the Mechanism of the Xingnaojing Injection against Ischemic Stroke Using a Combined Network Pharmacology Approach and Gut Microbiota Analysis. Evid Based Complement Alternat Med. 2022,2022:5886698. doi: 10.1155/2022/5886698
    Read this paper
  • Wu KH, Li JP, Chao WR, et al. Immunomodulation via MyD88-NFκB Signaling Pathway from Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury. Int J Mol Sci. 2022,23(10). doi: 10.3390/ijms23105295
    Read this paper
  • Yen IC, Tu QW, Chang TC, et al. 4-Acetylantroquinonol B ameliorates nonalcoholic steatohepatitis by suppression of ER stress and NLRP3 inflammasome activation. Biomed Pharmacother. 2021,138:111504. doi: 10.1016/j.biopha.2021.111504
    Read this paper
  • Karunarathne WAHM, Molagoda IMN, Choi YH, et al. Bisphenol A: A potential Toll-like receptor 4/myeloid differentiation factor 2 complex agonist. Environ Pollut. 2021,278:116829. doi: 10.1016/j.envpol.2021.116829
    Read this paper
  • Wu KH, Cheng CC, Li JP, et al. Toll-like receptor signalling associated with immunomodulation of umbilical cord-derived mesenchymal stem cells in mice with systemic lupus erythematosus. Lupus. 2020,29(2):165-175. doi: 10.1177/0961203319898532
    Read this paper
  • Lee SC, Hsu JS, Li CC, et al. Protective effect of leaf essential oil from Cinnamomum osmophloeum Kanehira on endotoxin-induced intestinal injury in mice associated with suppressed local expression of molecules in the signaling pathways of TLR4 and NLRP3. PLoS One. 2015,10(3):e0120700. doi: 10.1371/journal.pone.0120700
    Read this paper
  • Liu YH, Li XY, Chen CY, et al. Omega-3 fatty acid intervention suppresses lipopolysaccharide-induced inflammation and weight loss in mice. Mar Drugs. 2015,13(2):1026-36. doi: 10.3390/md13021026
    Read this paper
  • Dvoriantchikova G, Santos AR, Danek D, et al. The TIR-domain-containing adapter inducing interferon-β-dependent signaling cascade plays a crucial role in ischemia-reperfusion-induced retinal injury, whereas the contribution of the myeloid differentiation primary response 88-dependent signaling cascade is not as pivotal. Eur J Neurosci. 2014,40(3):2502-12. doi: 10.1111/ejn.12603
    Read this paper

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Figure 1. Western blot analysis of MYD88 using anti-MYD88 antibody (A00025-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # A00025-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 37 kDa. The expected band size for MYD88 is at 37 kDa.
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