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ProductsBack/MYD88 Mouse Monoclonal Antibody [Clone ID: OTI1A10]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MYD88 (Cat# RC202253, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88(Cat# TA502116). Positive lysates [LY432175] (100ug) and [LC432175] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MYD88 (Cat# RC202253, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88(Cat# TA502116). Positive lysates [LY432175] (100ug) and [LC432175] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MYD88 (Cat# RC202253, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88(Cat# TA502116). Positive lysates [LY432175] (100ug) and [LC432175] (20ug) can be purchased separately from OriGene.

MYD88 Mouse Monoclonal Antibody [Clone ID: OTI1A10]

CF502116
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMYD88
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MYD88 Mouse Monoclonal Antibody [Clone ID: OTI1A10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1A10
  • Gene ID4615
  • Target name
    MYD88
  • Target description
    MYD88 innate immune signal transduction adaptor
  • Target synonyms
    IMD68, MYD88D, WM1, myeloid differentiation primary response protein MyD88, TLR adaptor MYD88, mutant myeloid differentiation primary response 88, myeloid differentiation primary response 88, myeloid differentiation primary response gene (88)
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ99836
  • Protein Name
    Myeloid differentiation primary response protein MyD88
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MYD88 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MYD88 using anti-MYD88 antibody (A00025-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # A00025-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 37 kDa. The expected band size for MYD88 is at 37 kDa.
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