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ProductsBack/Myeloid Differentiation Factor 88 (MyD88) Polyclonal Antibody

Myeloid Differentiation Factor 88 (MyD88) Polyclonal Antibody

CAU25458
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetMyd88
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Overview

  • Supplier
    Biomatik
  • Product Name
    Myeloid Differentiation Factor 88 (MyD88) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID17874
  • Target name
    Myd88
  • Target description
    myeloid differentiation primary response gene 88
  • Target synonyms
    myeloid differentiation primary response protein MyD88, myeloid differentiation primary response protein 88
  • Host
    Rabbit
  • Protein IDP22366
  • Protein Name
    Myeloid differentiation primary response protein MyD88
  • Scientific Description
    The Myeloid Differentiation Factor 88 (MyD88) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MyD88 using anti-MyD88 antibody (A00025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MyD88 antigen affinity purified polyclonal antibody (Catalog # A00025-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MyD88 at approximately 33KD. The expected band size for MyD88 is at 33KD.
Product group Antibodies
Boster Bio
Anti-MyD88 Antibody Picoband(r)A00025-1-CARRIER-FREE
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetMyd88
  • SizePrice
Molt-4 lysates probed with MyD88 Polyclonal Antibody, Unconjugated (bs-1047R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
MyD88 Polyclonal AntibodyBS-1047R
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMyd88
  • SizePrice