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Western Blot Positive WB detected in: 293 whole cell lysate, Rat Brain tissue All lanes: MYH10 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 229, 231, 232, 233 kDa Observed band size: 250 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, Rat Brain tissue All lanes: MYH10 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 229, 231, 232, 233 kDa Observed band size: 250 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, Rat Brain tissue All lanes: MYH10 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 229, 231, 232, 233 kDa Observed band size: 250 kDa

MYH10 Antibody

CSB-PA015290LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetMYH10
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYH10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4628
  • Target name
    MYH10
  • Target description
    myosin heavy chain 10
  • Target synonyms
    NMMHC-IIB, NMMHCB, myosin-10, myosin heavy chain, nonmuscle type B, myosin, heavy chain 10, non-muscle, myosin, heavy polypeptide 10, non-muscle, nonmuscle myosin II heavy chain-B, nonmuscle myosin heavy chain IIB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35580
  • Protein Name
    Myosin-10
  • Scientific Description
    Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of non-muscle Myosin IIB/MYH10 using anti-non-muscle Myosin IIB/MYH10 antibody (A02681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates, Lane 4: human SKOV3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-non-muscle Myosin IIB/MYH10 antigen affinity purified polyclonal antibody (Catalog # A02681-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for non-muscle Myosin IIB/MYH10 at approximately 229KD. The expected band size for non-muscle Myosin IIB/MYH10 is at 229KD.
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