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MYO1B Antibody (YA3284, Rabbit)

HY-P83539
MedChem Express
TargetMYO1B
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MYO1B Antibody (YA3284, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4430
  • Target name
    MYO1B
  • Target description
    myosin IB
  • Target synonyms
    MMI-alpha, MMIa, MYH-1c, myr1, unconventional myosin-Ib, MYO1B variant protein, myosin-I alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MYO1B Antibody (YA3284) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYO1B.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MYO1B using anti-MYO1B antibody (A07011-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYO1B antigen affinity purified polyclonal antibody (Catalog # A07011-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYO1B at approximately 124 kDa. The expected band size for MYO1B is at 132 kDa.
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