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MYORG Antibody, Biotin conjugated

CSB-PA764657LD01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetMYORG
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYORG Antibody, Biotin conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID57462
  • Target name
    MYORG
  • Target description
    myogenesis regulating glycosidase
  • Target synonyms
    IBGC7, KIAA1161, NET37, myogenesis-regulating glycosidase, myogenesis regulating glycosidase (putative)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6NSJ0
  • Protein Name
    Myogenesis-regulating glycosidase
  • Scientific Description
    Putative glycosidase. Promotes myogenesis by activating AKT signaling through the maturation and secretion of IGF2.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYORG using anti-MYORG antibody (A32480). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYORG antigen affinity purified polyclonal antibody (Catalog # A32480) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYORG at approximately 87 kDa. The expected band size for MYORG is at 81 kDa.
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