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Myosin Light Chain 2 Antibody

HY-P80235
MedChem Express
TargetMYL2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Myosin Light Chain 2 Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4633
  • Target name
    MYL2
  • Target description
    myosin light chain 2
  • Target synonyms
    cardiac myosin light chain 2; cardiac ventricular myosin light chain 2; CMH10; MFM12; MLC2; MLC-2; MLC-2s/v; MLC-2v; myosin regulatory light chain 2, ventricular/cardiac muscle isoform; myosin, light chain 2, regulatory, cardiac, slow; myosin, light polypeptide 2, regulatory, cardiac, slow; regulatory light chain of myosin; RLC of myosin; slow cardiac myosin regulatory light chain 2; truncated myosin light chain 2; ventricular myosin light chain 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Myosin Light Chain 2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 19 kDa, targeting to Myosin Light Chain 2. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myosin Light Chain 2/MLC-2V/MYL2 using anti-Myosin Light Chain 2/MLC-2V/MYL2 antibody (A03215-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: monkey heart tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myosin Light Chain 2/MLC-2V/MYL2 antigen affinity purified polyclonal antibody (Catalog # A03215-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myosin Light Chain 2/MLC-2V/MYL2 at approximately 19 kDa. The expected band size for Myosin Light Chain 2/MLC-2V/MYL2 is at 19 kDa.
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