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ProductsBack/Myosin Light Chain 4, Alkali, Atrial, Embryonic (MYL4) Polyclonal Antibody

Myosin Light Chain 4, Alkali, Atrial, Embryonic (MYL4) Polyclonal Antibody

CAU23344
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetMYL4
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Overview

  • Supplier
    Biomatik
  • Product Name
    Myosin Light Chain 4, Alkali, Atrial, Embryonic (MYL4) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4635
  • Target name
    MYL4
  • Target description
    myosin light chain 4
  • Target synonyms
    ALC1; AMLC; atrial myosin light chain 1; GT1; myosin light chain 4; myosin, atrial/fetal muscle, light chain; myosin, light chain 4, alkali; atrial, embryonic; myosin, light polypeptide 4, alkali; atrial, embryonic; PRO1957
  • Host
    Rabbit
  • Protein IDP12829
  • Protein Name
    Myosin light chain 4
  • Scientific Description
    The Myosin Light Chain 4, Alkali, Atrial, Embryonic (MYL4) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYL4 using anti-MYL4 antibody (A08496-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL4 antigen affinity purified polyclonal antibody (Catalog # A08496-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL4 at approximately 24 kDa. The expected band size for MYL4 is at 22 kDa.
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