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Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

N-cadherin Polyclonal Antibody

BS-1172R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCDH2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    N-cadherin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1000
  • Target name
    CDH2
  • Target description
    cadherin 2
  • Target synonyms
    ACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); cadherin-2; calcium-dependent adhesion protein, neuronal; CD325; CDHN; CDw325; NCAD; N-cadherin 1; neural cadherin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19022
  • Protein Name
    Cadherin-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Cell Membrane-Coated Porphyrin Metal-Organic Frameworks for Cancer Cell Targeting and O2-Evolving Photodynamic Therapy. Zhang D et al., 2019 Oct 30, ACS Appl Mater Interfaces
    Read more
  • MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-kappaB system-mediated epithelial-mesenchymal transition. Ma T et al., 2019 Nov, Naunyn Schmiedebergs Arch Pharmacol
    Read more
  • Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B. Zhou S et al., 2019 Jun 30, Biochem Biophys Res Commun
    Read more
  • MFAP5 promotes basal-like breast cancer progression by activating the EMT program. Wu Y et al., 2019, Cell Biosci
    Read more
  • LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/beta-catenin pathway and predicts poor survival of glioma patients. Zhang J et al., 2018 Sep 12, J Exp Clin Cancer Res
    Read more
  • Long Non-Coding RNA LINC01260 Inhibits the Proliferation, Migration and Invasion of Spinal Cord Glioma Cells by Targeting CARD11 Via the NF-kappaB Signaling Pathway. Wu DM et al., 2018, Cell Physiol Biochem
    Read more
  • lncRNA H19 is involved in TGF-beta1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway. Yang W et al., 2017, PeerJ
    Read more
  • Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. Wang Q et al., 2016 Nov 8, Sci Rep
    Read more
  • Interaction-tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation. Park H et al., 2017 Jan, J Biomed Mater Res A
    Read more
  • The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. Fei F et al., 2015 Dec 24, J Exp Clin Cancer Res
    Read more

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Figure 1. Western blot analysis of CDH2 using anti-CDH2 antibody (A01577-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # A01577-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140KD. The expected band size for CDH2 is at 100KD.
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