
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
N-cadherin Polyclonal Antibody
BS-1172R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetCDH2
Overview
- SupplierBioss
- Product NameN-cadherin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1000
- Target nameCDH2
- Target descriptioncadherin 2
- Target synonymsACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD, cadherin-2, N-cadherin 1, cadherin 2, type 1, N-cadherin (neuronal), calcium-dependent adhesion protein, neuronal, neural cadherin
- HostRabbit
- IsotypeIgG
- Protein IDP19022
- Protein NameCadherin-2
- ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC41116161
References
- Cell Membrane-Coated Porphyrin Metal-Organic Frameworks for Cancer Cell Targeting and O2-Evolving Photodynamic Therapy. Zhang D et al., 2019 Oct 30, ACS Appl Mater InterfacesRead this paper
- MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-kappaB system-mediated epithelial-mesenchymal transition. Ma T et al., 2019 Nov, Naunyn Schmiedebergs Arch PharmacolRead this paper
- Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B. Zhou S et al., 2019 Jun 30, Biochem Biophys Res CommunRead this paper
- MFAP5 promotes basal-like breast cancer progression by activating the EMT program. Wu Y et al., 2019, Cell BiosciRead this paper
- LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/beta-catenin pathway and predicts poor survival of glioma patients. Zhang J et al., 2018 Sep 12, J Exp Clin Cancer ResRead this paper
- Long Non-Coding RNA LINC01260 Inhibits the Proliferation, Migration and Invasion of Spinal Cord Glioma Cells by Targeting CARD11 Via the NF-kappaB Signaling Pathway. Wu DM et al., 2018, Cell Physiol BiochemRead this paper
- lncRNA H19 is involved in TGF-beta1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway. Yang W et al., 2017, PeerJRead this paper
- Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. Wang Q et al., 2016 Nov 8, Sci RepRead this paper
- Interaction-tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation. Park H et al., 2017 Jan, J Biomed Mater Res ARead this paper
- The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. Fei F et al., 2015 Dec 24, J Exp Clin Cancer ResRead this paper









![Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_20180810_WB_KO_watermark_w_23060500_602.webp)