Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
N-cadherin Polyclonal Antibody
BS-1172R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat, Zebra Fish
TargetCDH2
Overview
- SupplierBioss Antibodies
- Product NameN-cadherin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1000
- Target nameCDH2
- Target descriptioncadherin 2
- Target synonymsACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); cadherin-2; calcium-dependent adhesion protein, neuronal; CD325; CDHN; CDw325; NCAD; N-cadherin 1; neural cadherin
- HostRabbit
- IsotypeIgG
- Protein IDP19022
- Protein NameCadherin-2
- ReactivityBovine, Human, Mouse, Porcine, Rat, Zebra Fish
- Storage Instruction-20°C
- UNSPSC12352203
References
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- The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. Fei F et al., 2015 Dec 24, J Exp Clin Cancer ResRead more