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IHC-P analysis of human placenta tissue section using GTX03240 N-WASP antibody [GT1328]. Dilution : 1:100
IHC-P analysis of human placenta tissue section using GTX03240 N-WASP antibody [GT1328]. Dilution : 1:100
IHC-P analysis of human placenta tissue section using GTX03240 N-WASP antibody [GT1328]. Dilution : 1:100

N-WASP antibody [GT1328]

GTX03240
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWASL
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Overview

  • Supplier
    GeneTex
  • Product Name
    N-WASP antibody [GT1328]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1328
  • Concentration
    1.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8976
  • Target name
    WASL
  • Target description
    WASP like actin nucleation promoting factor
  • Target synonyms
    N-WASP, NWASP, WASPB, actin nucleation-promoting factor WASL, Wiskott-Aldrich syndrome like, neural Wiskott-Aldrich syndrome protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00401
  • Protein Name
    Actin nucleation-promoting factor WASL
  • Scientific Description
    This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of N WASP/WASL using anti-N WASP/WASL antibody (A05438-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human HELA whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat liver tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysayes, Lane 10: mouse lung tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N WASP/WASL antigen affinity purified polyclonal antibody (Catalog # A05438-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for N WASP/WASL at approximately 70KD. The expected band size for N WASP/WASL is at 70KD.
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