N2,N2-Dimethylguanosine

MedChem Express

N2,N2-Dimethylguanosine [2140-67-2]

5

2140-67-2

Research Use Only

99.40

C12H17N5O5

311.29

N2,N2-Dimethylguanosine is a methylated nucleoside present in RNA and a structural modification component of tRNA. N2,N2-Dimethylguanosine hinders reverse transcriptase-mediated cDNA synthesis and is one of the key modifications that affect sequencing efficiency in high-throughput RNA sequencing. N2,N2-Dimethylguanosine can selectively remove one of the methyl groups through AlkB mutant enzymes (such as D135S/L118V) and convert it into N2-methylguanosine, thereby reducing the hindrance to reverse transcription[1][2].

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N(C=N2)C3=C2C(NC(N(C)C)=N3)=O)O1

2°C to 8°C

12352200