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Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody
Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody
Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody

N4BP1 Antibody

CSB-PA003360
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetN4BP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    N4BP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9683
  • Target name
    N4BP1
  • Target description
    NEDD4 binding protein 1
  • Target synonyms
    NEDD4-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75113
  • Protein Name
    NEDD4-binding protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of N4BP1 using anti-N4BP1 antibody (A12575-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N4BP1 antigen affinity purified polyclonal antibody (Catalog # A12575-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for N4BP1 at approximately 110 kDa. The expected band size for N4BP1 is at 100 kDa.
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