N6-Propargyl-adenosine

Jena Bioscience

N6-Propargyl-adenosine

5

Research Use Only

≥95%

C13H15N5O4

305.29 g/mol

N6-propargyl-adenosine (N6pA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. N6pA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I,II and III and posttranscriptionally by poly(A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

c1(c2c(ncn1)n(cn2)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O)NCC#C

-20°C

12352200