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Western Blot analysis of RAW264.7 cells using NAB2 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using NAB2 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using NAB2 Polyclonal Antibody

NAB2 Antibody

CSB-PA003366
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNAB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NAB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4665
  • Target name
    NAB2
  • Target description
    NGFI-A binding protein 2
  • Target synonyms
    MADER, NGFI-A-binding protein 2, EGR1 binding protein 2, melanoma-associated delayed early response protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15742
  • Protein Name
    NGFI-A-binding protein 2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NAB2 using anti-NAB2 antibody (A02873-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAB2 antigen affinity purified polyclonal antibody (A02873-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAB2 at approximately 57 kDa. The expected band size for NAB2 is at 57 kDa.
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