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Western Blot Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue All lanes: NADK2 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 46, 32 kDa Observed band size: 50, 46 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue All lanes: NADK2 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 46, 32 kDa Observed band size: 50, 46 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue All lanes: NADK2 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 46, 32 kDa Observed band size: 50, 46 kDa

NADK2 Antibody

CSB-PA676825LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNADK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NADK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID133686
  • Target name
    NADK2
  • Target description
    NAD kinase 2, mitochondrial
  • Target synonyms
    C5orf33, DECRD, MNADK, NADKD1, NAD kinase 2, mitochondrial, NAD kinase domain-containing protein 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4G0N4
  • Protein Name
    NAD kinase 2, mitochondrial
  • Scientific Description
    Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C5orf33/NADK2 using anti-C5orf33/NADK2 antibody (A09619-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5orf33/NADK2 antigen affinity purified polyclonal antibody (Catalog # A09619-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5orf33/NADK2 at approximately 53 kDa. The expected band size for C5orf33/NADK2 is at 49 kDa.
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