NAD/NADH Assay Kit (Fluorometric)

LifeSpan BioSciences

NAD/NADH Assay Kit (Fluorometric)

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For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Direct NAD/NADH Assays in 96-Well Plate

Spectrophotometry (fluorometric) For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Direct NAD/NADH Assays in 96-Well Plate

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Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. This NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lambda ex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient Method to measure NAD, NADH and their ratio.Direct NAD/NADH Assays in 96-Well PlateNEW!!!;

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