NADP/NADPH Assay Kit (Fluorometric)

LifeSpan BioSciences

NADP/NADPH Assay Kit (Fluorometric)

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For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

Spectrophotometry (fluorometric) For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

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Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. This NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lambda ex/em = 530/585 nm, is proportional to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and with minimal interference (<1%) by NAD+/NADH. Our assay is a convenient Method to measure NADP+, NADPH and their ratio.

-20°C

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