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WB analysis of mouse brain tissue lysate using GTX33343 NAK / TBK1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse brain tissue lysate using GTX33343 NAK / TBK1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse brain tissue lysate using GTX33343 NAK / TBK1 antibody. Dilution : 1:1000 Loading : 25microg per lane

NAK / TBK1 antibody

GTX33343
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTBK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NAK / TBK1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29110
  • Target name
    TBK1
  • Target description
    TANK binding kinase 1
  • Target synonyms
    FTDALS4; IIAE8; NAK; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine-protein kinase TBK1; T2K
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHD2
  • Protein Name
    Serine/threonine-protein kinase TBK1
  • Scientific Description
    The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (A00261-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human LNCAP whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAK/TBK1 antigen affinity purified polyclonal antibody (Catalog # A00261-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAK/TBK1 at approximately 84 kDa. The expected band size for NAK/TBK1 is at 84 kDa.
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