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ProductsBack/Nanog antibody [GT3312]
Sample (30 μg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Nanog antibody [GT3312]

GTX627421
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNANOG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Nanog antibody [GT3312]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT3312
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79923
  • Target name
    NANOG
  • Target description
    Nanog homeobox
  • Target synonyms
    homeobox protein NANOG, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9H9S0
  • Protein Name
    Homeobox protein NANOG
  • Scientific Description
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5-TAAT[GT][GT]-3 or 5-[CG][GA][CG]C[GC]ATTAN[GC]-3 (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ye P, Chi X, Yan X, et al. Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells. Biomolecules. 2022,12(5). doi: 10.3390/biom12050668
    Read this paper
  • Hassan G, Zahra MH, Seno A, et al. The significance of ErbB2/3 in the conversion of induced pluripotent stem cells into cancer stem cells. Sci Rep. 2022,12(1):2711. doi: 10.1038/s41598-022-04980-y
    Read this paper
  • Ciarpella F, Zamfir RG, Campanelli A, et al. Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity. iScience. 2021,24(12):103438. doi: 10.1016/j.isci.2021.103438
    Read this paper
  • Biagioni A, Chillà A, Del Rosso M, et al. CRISPR/Cas9 uPAR Gene Knockout Results in Tumor Growth Inhibition, EGFR Downregulation and Induction of Stemness Markers in Melanoma and Colon Carcinoma Cell Lines. Front Oncol. 2021,11:663225. doi: 10.3389/fonc.2021.663225
    Read this paper
  • Walker SJ, Wagoner AL, Leavitt D, et al. A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines. Heliyon. 2021,7(4):e06617. doi: 10.1016/j.heliyon.2021.e06617
    Read this paper
  • Merle C, Lagarde P, Lartigue L, et al. Acquisition of cancer stem cell capacities after spontaneous cell fusion. BMC Cancer. 2021,21(1):241. doi: 10.1186/s12885-021-07979-2
    Read this paper
  • Chen YL, Yen YC, Jang CW, et al. Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells. Sci Rep. 2021,11(1):644. doi: 10.1038/s41598-020-80060-3
    Read this paper
  • Lee KY, Kuo TC, Chou CM, et al. Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling. Cells. 2020,10(1). doi: 10.3390/cells10010028
    Read this paper
  • Jao TM, Fang WH, Ciou SC, et al. PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer. Cancer Lett. 2021,499:290-300. doi: 10.1016/j.canlet.2020.11.017
    Read this paper
  • Blanas A, Zaal A, van der Haar Àvila I, et al. FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State. Cancers (Basel). 2020,12(9). doi: 10.3390/cancers12092580
    Read this paper

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