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NAP1L1 Antibody (YA2492, Rabbit)

HY-P82747
MedChem Express
TargetNAP1L1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NAP1L1 Antibody (YA2492, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4673
  • Target name
    NAP1L1
  • Target description
    nucleosome assembly protein 1 like 1
  • Target synonyms
    NAP1, NAP1L, NRP, nucleosome assembly protein 1-like 1, HSP22-like protein interacting protein, NAP-1-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NAP1L1 Antibody (YA2492) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2492), targeting NAP1L1, with a predicted molecular weight of 45 kDa (observed band size: 52,60 kDa). NAP1L1 Antibody (YA2492) can be used for WB, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NAP1L1 using anti-NAP1L1 antibody (A05100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP1L1 antigen affinity purified polyclonal antibody (Catalog # A05100-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAP1L1 at approximately 55 kDa. The expected band size for NAP1L1 is at 45 kDa.
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