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WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32154 NAP1L5 antibody. Working concentration : 1 μg/ml
WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32154 NAP1L5 antibody. Working concentration : 1 μg/ml
WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32154 NAP1L5 antibody. Working concentration : 1 μg/ml

NAP1L5 antibody

GTX32154
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNAP1L5
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Overview

  • Supplier
    GeneTex
  • Product Name
    NAP1L5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID266812
  • Target name
    NAP1L5
  • Target description
    nucleosome assembly protein 1 like 5
  • Target synonyms
    DRLM, nucleosome assembly protein 1-like 5, down-regulated in liver malignancy
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NT1
  • Protein Name
    Nucleosome assembly protein 1-like 5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NAP1L5 using anti-NAP1L5 antibody (A14492-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP1L5 antigen affinity purified polyclonal antibody (Catalog # A14492-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAP1L5 at approximately 19 kDa. The expected band size for NAP1L5 is at 19 kDa.
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