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NAPE-PLD Antibody (Biotin)

LS-C398792
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetNAPEPLD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NAPE-PLD Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID222236
  • Target name
    NAPEPLD
  • Target description
    N-acyl phosphatidylethanolamine phospholipase D
  • Target synonyms
    C7orf18; FMP30; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; NAPE-hydrolyzing phospholipase D; NAPE-PLD
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse kidney lysates probed with NAPE-PLD Polyclonal Antibody, Unconjugated (bs-19015R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPEPLD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPEPLD.
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WB analysis of 293T cells using GTX45154 NAPE-PLD antibody at 0.2-1microg/ml.
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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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Western blot All lanes: NAPEPLD antibody at 6microg/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse kidney tissue Lane 3: Mouse stomach tissue Lane 4: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa
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