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Western blot analysis of extracts of various cell lines using NAPRT Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using NAPRT Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using NAPRT Polyclonal Antibody at dilution of 1:3000.

NAPRT Polyclonal Antibody

E-AB-64080
Elabscience
Product group Antibodies

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Overview

  • Supplier
    Elabscience
  • Product Name
    NAPRT Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Nicotinic acid (NA) is a coenzyme in cellular redox reactions,and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and,thus,to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN),which is then converted to NA adenine dinucleotide (NaAD),and finally to nicotinamide adenine dinucleotide (NAD).
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NAPRT using anti-NAPRT antibody (A10553-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAPRT antigen affinity purified polyclonal antibody (Catalog # A10553-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAPRT at approximately 58 kDa. The expected band size for NAPRT is at 58 kDa.
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