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FACS analysis of PFA-fixed Kelly cells using GTX04238 NAT8L antibody. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10microg/ml
FACS analysis of PFA-fixed Kelly cells using GTX04238 NAT8L antibody. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10microg/ml
FACS analysis of PFA-fixed Kelly cells using GTX04238 NAT8L antibody. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10microg/ml

NAT8L antibody

GTX04238
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNAT8L
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Overview

  • Supplier
    GeneTex
  • Product Name
    NAT8L antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. ICC/IF: 10microg/ml. FACS: 10microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID339983
  • Target name
    NAT8L
  • Target description
    N-acetyltransferase 8 like
  • Target synonyms
    camello-like protein 3; CML3; NAA synthetase; NACED; N-acetylaspartate synthetase; N-acetyltransferase 8-like (GCN5-related, putative); NAT8-LIKE; Shati
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N9F0
  • Protein Name
    N-acetylaspartate synthetase
  • Scientific Description
    This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. The gene mutation results in primary NAA deficiency (hypoacetylaspartia). [provided by RefSeq, Dec 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of U20S cells using anti-NAT8L antibody (A07393-2). Overlay histogram showing U20S cells stained with A07393-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAT8L Antibody (A07393-2, 1microg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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