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Natriuretic Peptide Receptor A Polyclonal Antibody

BS-10405R
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetNPR1
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Overview

  • Supplier
    Bioss
  • Product Name
    Natriuretic Peptide Receptor A Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4881
  • Target name
    NPR1
  • Target description
    natriuretic peptide receptor 1
  • Target synonyms
    ANPRA, ANPa, GUC2A, GUCY2A, NPRA, atrial natriuretic peptide receptor 1, ANP-A, ANPR-A, GC-A, NPR-A, atrial natriuretic peptide receptor type A, atrionatriuretic peptide receptor A, guanylate cyclase A, natriuretic peptide A type receptor, natriuretic peptide receptor A, natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A), testicular tissue protein Li 20
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16066
  • Protein Name
    Atrial natriuretic peptide receptor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NPR1 using anti-NPR1 antibody (A01042). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPR1 antigen affinity purified polyclonal antibody (Catalog # A01042) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPR1 at approximately 119KD. The expected band size for NPR1 is at 119KD.
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