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Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCAM antibody (GTX111684) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCAM antibody (GTX111684) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCAM antibody (GTX111684) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

NCAM antibody

GTX111684
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNCAM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NCAM antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4684
  • Target name
    NCAM1
  • Target description
    neural cell adhesion molecule 1
  • Target synonyms
    CD56, MSK39, NCAM, neural cell adhesion molecule 1, antigen recognized by monoclonal antibody 5.1H11, neural cell adhesion molecule, NCAM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13591
  • Protein Name
    Neural cell adhesion molecule 1
  • Scientific Description
    This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCAM1 using anti-NCAM1 antibody (A00184-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 antigen affinity purified polyclonal antibody (Catalog # A00184-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAM1 at approximately 150KD. The expected band size for NCAM1 is at 150KD.
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FACS analysis of human peripheral blood lymphocytes using GTX01477-02 NCAM antibody [MY31] (Biotin). Right panel : co-stained with NCAM antibody [MY31] (Biotin) and Human CD3 antibody (APC) Left panel : co-stained with isotype control and Human CD3 antibody (APC) antibody amount : 0.5 μg (5 μl)
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FACS analysis of human peripheral blood lymphocytes using GTX01477-06 NCAM antibody [MY31] (FITC). Right panel : co-stained with NCAM antibody [MY31] (FITC) and Human CD3 antibody (APC) Left panel : co-stained with isotype control and Human CD3 antibody (APC) antibody amount : 1 μg (5 μl)
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FACS analysis of human peripheral blood lymphocytes using GTX01477-07 NCAM antibody [MY31] (APC). Right panel : co-stained with NCAM antibody [MY31] (APC) and Human CD3 antibody (FITC) Left panel : co-stained with isotype control and Human CD3 antibody (FITC) antibody amount : 0.5 μg (5 μl)
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FACS analysis of human peripheral blood lymphocytes using GTX01477-08 NCAM antibody [MY31] (PE). Right panel : co-stained with NCAM antibody [MY31] (PE) and Human CD3 antibody (APC) Left panel : co-stained with isotype control and Human CD3 antibody (APC) antibody amount : 1 μg (5 μl)
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