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Western Blot analysis of COLO cells using hCAP-H Polyclonal Antibody
Western Blot analysis of COLO cells using hCAP-H Polyclonal Antibody
Western Blot analysis of COLO cells using hCAP-H Polyclonal Antibody

NCAPH Antibody

CSB-PA008364
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNCAPH
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Overview

  • Supplier
    Cusabio
  • Product Name
    NCAPH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23397
  • Target name
    NCAPH
  • Target description
    non-SMC condensin I complex subunit H
  • Target synonyms
    BRRN1, CAP-H, CAPH, MCPH23, NCAPH1, condensin complex subunit 2, barren homolog 1, chromosome-associated protein H
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15003
  • Protein Name
    Condensin complex subunit 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCAPH using anti-NCAPH antibody (A09559-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAPH antigen affinity purified polyclonal antibody (Catalog # A09559-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAPH at approximately 100 kDa. The expected band size for NCAPH is at 83-100 kDa.
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